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Shahi, N.
- Leukocyte Response and Phagocytic Activity in Common Carp, Cyprinus Carpio Experimentally Infected with Virulent Aeromonas Allosaccharophila
Abstract Views :919 |
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Authors
Affiliations
1 Directorate of Coldwater Fisheries Research (ICAR), Bhimtal 263 136, Nainital, (UK), IN
1 Directorate of Coldwater Fisheries Research (ICAR), Bhimtal 263 136, Nainital, (UK), IN
Source
Journal of Ecophysiology and Occupational Health, Vol 14, No 1-2 (2014), Pagination: 66-70Abstract
Total and differential leukocyte count, and the phagocytic activity in blood of common carp, Cyprinus carpio (Linnaeus, 1758), experimentally infected with virulent Aeromonas allosaccharophila was evaluated in this study. For experimental study, common carp fingerlings (10.0 ± 1.5 g Weight, 8.5 ± 2.3 cm Length) were divided into five groups in duplicates of un-injected fish, fish injected with sterile phosphate buffered saline (PBS), and fish injected with 4.2 x 104, 1.9 x 106 and 7.3 x 108 colony forming units per milliliter (CFU ml-1) of suspension of A. allosaccharophila. Forty eight hours after infection, the total and differential leukocyte count and phagocytic activity of blood leukocytes of common carp was determined. The leukocyte, lymphocyte and monocyte count were significantly (P< 0.05) high in experimentally infected fishes, while no significant change in neutrophils number was observed. In fishes injected with 4.2 x 104, 1.9 x 106 and 7.3 x 108 CFU ml-1 of bacterial suspension, the percentage of phagocytic activity in the blood were 55.3, 54.7 and 58.2, respectively. The result suggests that, A. allosaccharophila could influence the leukocyte number and phagocytic activity of blood of common carp, which is an integral component of innate immune system of fish.Keywords
Bacteria, Blood, Fish, Innate Immunity, Phagocytosis.- Corneal Hypertrophy in Farmed Chocolate Mahseer, Neolissochilus Hexagonolepis, from (uttarakhand), India
Abstract Views :949 |
PDF Views:227
Authors
Affiliations
1 Directorate of Coldwater Fisheries Research, (ICAR) Industrial Area, Bhimtal, 263 136, Nainital, (Uttarakhand), IN
2 Indian Veterinary Research Institute, Izatnagar, Bareilly, (UP), IN
1 Directorate of Coldwater Fisheries Research, (ICAR) Industrial Area, Bhimtal, 263 136, Nainital, (Uttarakhand), IN
2 Indian Veterinary Research Institute, Izatnagar, Bareilly, (UP), IN
Source
Journal of Ecophysiology and Occupational Health, Vol 14, No 1-2 (2014), Pagination: 79-82Abstract
Corneal hypertrophy was observed in fingerlings of chocolate mahseer, Neolissochilus hexagonolepis (McClelland, 1839) from Uttarakhand, India. The advanced stage of infection was marked with damage of cornea and shrunken eye. Skeletal deformity was also recorded in some of the infected fishes. Samples of eye and other organ were collected for histopathological, parasite and microbial investigation. Histological examination of the eye indicated ocular collapse, entirely damaged cornea, disruption of vitreous, aphakic globe, oedema of choroid and optic nerve and, deformed retina. Microscopic observations of wet mounts and histopathology ruled out infectious agents like microsporidia, myxosporidea, cystercoidiasis, atypical mycobacteria, digeneans and fungi. PCR for viral aetiology did not yield any significant amplicons. Further detail study related to physic-chemical parameter need to be carried out to ascertain the exact cause of this disease.Keywords
neolissochilus Hexagonolepis, Pathology, Eye, Corneal Hypertrophy, Chocolate Mahseer.- Gonadal Development Stages of Wild Male Golden Mahseer, Tor putitora from Nainital Region of Uttarakhand, India
Abstract Views :848 |
PDF Views:287
Authors
Affiliations
1 Industrial Area, Bhimtal, Uttarakhand-263 136, IN
1 Industrial Area, Bhimtal, Uttarakhand-263 136, IN